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A0759-10D Mouse Anti-Actin, pan (alpha, beta, gamma) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG
Clone Number
11C68
Grade
Affinity Purified
Applications
IF
Crossreactivity
Bo Ca Ch Fr Gt Gp Hm Hu Mo Po Rb Rt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are ~90% conserved, except in the N-terminal 18aa (50-60%homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.

Applications
Suitable for use in Immunofluorescence. Other applications not tested.
Recommended Dilutions
Immunofluorescence: 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Immunogen
Synthetic peptide corresponding to 11aa SGPSIVHRKCF near the C-terminal of human actin.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC)
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Actin. Species Crossreactivity: bovine, sheep, goat, porcine, rabbit, canine, mouse, rat, guinea pig, hamster, chicken, carp, frog and snail.
References
1. Ohmuri H (1995) Gene Accession # S38782. 2. Vandekerchove, J et al (1978) Eur. J. Biochem. 90, 451. 3. Lessard J et al (1988) Cell. Motil Cytoskel. 10, 349. 4. North JA et al (1994) J. Cell Sci. 107, 437.
USBio References
No references available
Conjugates
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