Applications
Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1ug/ml using lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, or HEK293T human embryonic kidney cell line Neutralization of receptor-ligand interaction: 0.5-2ug/ml Blocks 50% of the binding of 50ng/mL of rhCripto 1 to immobilized rhActRIB/Fc (100ul of 2ug/ml was coated in each well) in a functional ELISA. At 20ug/ml there will be >90% blocking KO Validation: Activin RIB/ALK‑4 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Activin RIB/ALK‑4 knockout HeLa cell line. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Leu32-Glu126 from human Activin RIB expressed in NS0 cells.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Activin RIB.