Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.
Applications
Suitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Neutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml recombinant human Activin A to immobilized recombinant human Act RIIA/Fc Chimera (100ul of a 0.5ng/ml solution was coated in each well) in a functional ELISA assay. Western Blot: 0.1ug/ml Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human prostate cancer tissue Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ser25-Asp82 from human Activin RIIA, expressed in Sf 21 cells.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug
Specificity
Recognizes human Activin Receptor Type IIA (RIIA). Shows <2% crossreactivity with recombinant human Activin RIIB.
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