Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.
Applications
Suitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Neutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml of recombinant human Activin A to immobilized recombinant human Act RIIB/Fc Chimera (100ul of a 2ug/ml solution was coated in each well) in an ELISA type assay. Western Blot: 0.1ug/ml, recombinant human Activin RIIB Fc Chimera Immunohistochemistry (Paraffin): 5-15ug/ml with immersion fixed paraffin-embedded sections of human placenta (cross-section of chorionic villi Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived, recombinant protein corresponding to Ser19-Thr134 from human Activin Receptor Type IIB extracellular domain.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by immunoaffinity chromatography. |Endotoxin: <0.1EU/ug (LAL)
Specificity
Recognizes human Activin Receptor Type IIB (RIIB). Shows <1% crossreactivity with recombinant human Activin RIIA, recombinant human Activin RIA and recombinant human Activin RIB.
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