Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, BMP-7, GDF-1, GDF-3, Myostatin (GDF-8), and GDF-11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.
Applications
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml. The detection limit for recombinant human Activin RIIB is ~20ng/lane under reducing and non-reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ser19-Thr134 from human Activin RIIB, expressed in NSO cells.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes recombinant human Activin RIIB. Shows ~5% crossreactivity with recombinant human Activin RIIA.