Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop- tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co- repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the α5β1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the α5β1 integrin (4).

Suitable for use in Western Blot. Other applications not tested.

Western Blot: 2-4ug’ml Optimal dilutions to be determined by the researcher.

293 cell lysate

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide AES antibody was raised against a 16 amino acid peptide from near the amino-terminus of human AES (Genbank accession No. NP_945320).

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.|

Purified by immunoaffinity chromatography.|

Recognizes human AES. Species Crossreactivity: mouse.