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A1059-89 Apoptosis, Human BioAssay™ Sample Kit

Specifications
References
Brand
BioAssay™
Specificity
Each antibody in the Apoptosis Sampler Kit detects endogenous levels of its respective protein. Cleaved Caspase-3 (Asp175), Cleaved Caspase-7 (Asp198) and Cleaved PARP (Asp214) Antibodies recognize only the large fragments of their respective cleaved proteins. Cleaved Caspase-9 (Asp330) Antibody recognizes either the 37kD (with prodomain) or 17kD large fragment of the cleaved protein. Caspase-3, Caspase-7, Caspase-9 and PARP Antibodies recognize both the full length and large cleaved fragments of their respective proteins.
Purity
Purified by Protein A and peptide affinity chromatography.
Kit Type
Apoptosis
Tests
84
Detection Method
Colorimetric/Fluorescent
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C

Apoptosis, or programmed cell death, is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 8, 9, 10 and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, alpha-Fodrin, DFF and Lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase. Proapoptotic stimuli include the FasL, TNF-alpha, DNA damage and ER stress. Fas and TNFR activate caspases 8 and 10; DNA damage leads to the activation of caspase-9; and ER stress leads to the calcium-mediated activation of caspase-12. The inhibitor of apoptosis protein (IAP) family includes XIAP and Survivin and functions by binding and inhibiting several caspases (4,5). Smac/Diablo, a mitochondrial protein, is released into the cytosol upon mitochondrial stress and competes with caspases for binding of IAPs. The interaction of Smac/Diablo with IAPs relieves the inhibitory effects of the IAPs on caspases (6).

Source
Human
Purity
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity
Each antibody in the Apoptosis Sampler Kit detects endogenous levels of its respective protein. Cleaved Caspase-3 (Asp175), Cleaved Caspase-7 (Asp198) and Cleaved PARP (Asp214) Antibodies recognize only the large fragments of their respective cleaved proteins. Cleaved Caspase-9 (Asp330) Antibody recognizes either the 37kD (with prodomain) or 17kD large fragment of the cleaved protein. Caspase-3, Caspase-7, Caspase-9 and PARP Antibodies recognize both the full length and large cleaved fragments of their respective proteins.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
(1) Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261–3270. (2) Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269–290. (3) Nakagawa, T. et al. (2000) Nature 403, 98–103. (4) Deveraux, Q.L. et al. (1998) EMBO J. 17, 2215–2223. (5) Li, F. et al. (1998) Nature 396, 580–584. (6) Du, C. et al. (2000) Cell 102, 33–42.
USBio References
No references available
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