Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry. Other applications not tested.

Flow Cytometry: 50ug/ml. This antibody was validated for flow cytometry using MCF-7 cells. For intracellular staining to detect Akt1, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 50ug/ml and add 10ul of the diluted solution to 1-5x10e5 cells in a total reaction volume not exceeding 20ulL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Immunocytochemistry: 8-25ug/ml to detect Akt1 in fixed human peripheral blood mononuclear cells. Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Sf 21-derived recombinant human v-akt murine thymoma viral oncogene homolog 1 aa2-480.

Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.

Purified by Protein G affinity chromatography.

Recognizes human Akt1. Does not detect rhAkt2 or rhAkt3.