Applications
Suitable for use in IEP, Single and Double RID. Other applications have not been tested.
Recommended Dilutions
IEP: use 2ul serum, plasma or equivalent against 120ul antiserum. Double RID: Use a rosette arrangement with 10ul antiserum in a 3 mm diameter centre well and 2ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. SIngle RID: use 0.5-1% antiserum in the gel. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Highly purified albumin isolated from hamster serum.
Form
Supplied as a lyophilized powder. No preservatives added. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
Specificity
Recognizes Hamster serum protein.