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A1372-25A Ames Medium w/L-Glutamine w/o Calcium Chloride, Magnesium Sulfate (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
4°C

Ames' medium was formulated to support retinal tissue in relatively short-term culture. Rabbit retina has been incubated in Ames' medium for over 2 days with its metabolism and electrical responses to light stimuli well maintained. This mixture is a medium of choice for maintaining central nervous system tissue in vitro.

The retina is an important in vitro model for the central nervous system. It is more readily accessible than most nervous tissue and is strong enough to remain intact during manipulations. The retina of the rabbit commonly contains no penetrating blood vessels. It is nurtured by diffusion from capillary networks on either side. For this reason, the tissue will survive and functions without circulating blood, as long as it is bathed in a medium that closely resembles in vivo conditions.
Appearance
White to pale yellow, homogeneous, free flowing powder
Solubility
Colorless to pale yellow, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Directions per Liter
Gas ~900ml of water (15-20°C) with 100% CO2. While gently stirring the water, add 1.9g sodium bicarbonate. Add 8.5g of the powder medium and stir until dissolved. Adjust the pH of the medium to 0.2-0.3 pH unit below the desired level using 1N NaOH and 1N HCl. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Inorganic Salts
Potassium Chloride0.231
Potassium Phosphate Mono.0.068
Sodium Chloride7.01
Amino Acids
L-Alanine0.0024
L-Arginine•HCl0.00421
L-Asparagine0.00084
L-Aspartic Acid0.00012
L-Cystine•2HCl0.000065
L-Glutamic Acid, Sodium0.001183
L-Glutamine0.073
Glycine0.00045
L-Histidine•HCl•H2O0.002513
L-Isoleucine0.00058
L-Leucine0.00144
L-Lysine•HCl0.003648
L-Methionine0.00039
L-Phenylalanine0.00132
L-Proline 0.00007
L-Serine0.00252
Taurine0.00075
L-Threonine0.00333
L-Tryptophan0.00049
L-Tyrosine•2Na•2H2O0.00211
L-Valine0.00176
Vitamins
Ascorbic Acid•Na0.01796
D-Biotin0.0001
Choline Chloride0.0007
Folic Acid 0.0001
myo-Inositol0.0272
Niacinamide0.0001
D-Pantothenic Acid•Ca0.0001
Pyridoxal•HCl0.0001
Riboflavin0.00001
Thiamine•HCl0.0001
Other
Cytidine0.00073
Thymidine0.00024
D-Glucose1.081
Hypoxanthine0.00082
Pyruvic Acid, Sodium0.01333
Uridine0.00073
Total:8.5g/Liter
References
1. Lipin MY and Vigh J. Quantifying the Effect of Light Activated Outer and Inner Retinal Inhibitory Pathways on Glutamate Release from Mixed Bipolar Cells. Synapse. 2018 Jan 23. doi: 10.1002/syn.22028. [Epub ahead of print].|2. Tooker RE and Vigh J. Light-evoked S-nitrosylation in the retina. J Comp Neurol. 2015. 523(14):2082-110. doi: 10.1002/cne.23780.|Other references: 1. Ames, A., Nesbett, F., J. Neurochem. 37: 837 (1981).|
USBio References
1. Lipin MY and Vigh J. Quantifying the Effect of Light Activated Outer and Inner Retinal Inhibitory Pathways on Glutamate Release from Mixed Bipolar Cells. Synapse. 2018 Jan 23. doi: 10.1002/syn.22028. [Epub ahead of print].|2. Tooker RE and Vigh J. Light-evoked S-nitrosylation in the retina. J Comp Neurol. 2015. 523(14):2082-110. doi: 10.1002/cne.23780.
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