Amies fomulated a medium for transport of microorganisms using a balanced salt solution. Amies Medium without Charcoal is a non-nutritional, phosphate buffered type medium used to maintian the viablilty of microorganisms without a significant increase in growth.
| Calcium Chloride | 0.1 |
| Magnesium Chloride | 0.1 |
| Potassium Chloride | 0.2 |
| Potassium Phosphate Mono | 0.2 |
| Sodium Chloride | 3 |
| Sodium Phosphate Dibasic | 1.15 |
| Sodium Thioglycolate | 1 |
| Total: | 5.75g/L |
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