AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively.
Applications
Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5- 1.0ug/ml detection limit for rmAMIGO3 is ~ 1ng/well. Western Blot: 1-2ug/ml detection limit for rmAMIGO3 is ~ 25ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 25ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived rmAMIGO3. (aa 20 - 378)
Form
Supplied as a lyophilized powder in 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse AMIGO3.