Aminoacylase 1 (ACY1) is an intracellular soluble homodimeric zinc-binding enzyme. It is involved in the breakdown of acetylated amino acids generated during protein degradation by catalyzing the formation of free aliphatic amino acids from N-acetylated precursors. This enzyme is evolutionarily conserved between mouse and human. Mutations in the human ACY1 gene might cause inborn metabolic disorders. The amino acid sequence of mouse ACY1 is 93% and 85% identical to that of rat and human/chimpanzee.
Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~2ng/well. Western Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~ 10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection willincrease sensitivity by 5to 50 fold. Immunoprecipitation: 25ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived recombinant corresponding to aa1-408 of mouse Aminoacylase 1 (Acession #Q99JW2)
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse Aminoacylase 1. Does not crossreact with human. Species Sequence Homology: rat; 93%, human and chimpanzee; 85%
TDS
NCBI
UniProt
Tech Support
Customer Service
