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A1580-03H Mouse Anti-AMPH (Amphiphysin, AMPH1)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG
Clone Number
3
Grade
Affinity Purified
Applications
IHC IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrinmediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.

Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to human Amphiphysin.
Form
Supplied as a liquid in PBS, 0.1mM PMSF, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human AMPH. Species sequence homology: Bovine, canine, hamster, monkey, mouse, rabbit, rat.
Conjugates
Pricing
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