Amylin is a 37aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism. The calcitonin family peptides probably act through G-protein coupled membrane receptors.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 1:12800 (0.0781ug/ml) Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
Lys-Cys-Asn-Thr-Ala-Thr-Cys-Ala-Thr-Gln-Arg-Leu-Ala Disulfide Bridge: Cys2-Cys7
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS/ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa1-13 of human Amylin.
Form
Supplied as a lyophilized powder from PBS. Reconstitute with 85ul sterile PBS and 15ul sterile ddH2O..
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Amylin.