Applications
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Immunofluorescence: 1:200 Immunohistochemistry (PAP or ABC): 1:500 Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
Lys-Cys-Asn-Thr-Ala-Thr-Cys-Ala-Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Val-Arg-Ser-Ser-Asn-Asn-Leu-Gly-Pro-Val-Leu-Pro-Pro-Thr-Asn-Val-Gly-Ser-Asn-Thr-Tyr-NH2 Disulfide Bridge: Cys2-Cys7
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to rat Amylin.
Form
Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.
Specificity
Recognizes rat and mouse Amylin. % Cross-reactivity with Peptides: Amylin amide (rat): 100%; Amylin amide (feline): 100%; Amyline amide (human): 0%; CGRP (human and rat): 0%; Pancreatic polypeptide, Amylin, Insulin, Pro-Insulin, Glucagon, Somatostatin-28 (all human): 0%; VIP (human, rat, porcine): 0%.