Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:12,800 (0.0781 ug/ml) Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
Lys-Cys-Asn-Thr-Ala-Thr-Cys-Ala-Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Val-Arg-Ser-Ser-Asn-Asn-Leu-Gly-Pro-Val-Leu-Pro-Pro-Thr-Asn-Val-Gly-Ser-Asn-Thr-Tyr-NH2 Disulfide Bridge: Cys2 - Cys7
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to rat Amylin.
Form
Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 400ul PBS.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes rat Amylin. Species Crossreactivity: mouse, human, feline; Amylin-Amide rat, human: 100%; Peptide Crossreactivity: Insulin, Proinsulin, Glucagon, Somatostatin 28, Pancreatic Polypeptide, CGRP, CGRP II, Growth Hormone: 0%.