Amyloid b (Ab) accumulates in the senile plaques of the brain during Alzheimer’s disease and during certain classes of cerebrovascular angiopathy. Ab generation is initiated by proteolytic cleavage of the Amyloid Precursor Protein (APP) by b-secretase (BACE) at a site which corresponds to the Ab N terminus. A second proteolytic cleavage by g-secretase at a site corresponding to the Ab C terminus releases the Ab peptide. The insoluble Ab peptide forms aggregates in the vessels and senile plaques of Alzheimer’s disease brains.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 0.1ug/ml Western Blot: 1ug/ml HRP/ECL detection. Recommended blocking with casein/Tween-20 based buffer. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to N-terminal Amyloid beta conjugated to KLH.
Form
Supplied as a lyophilized powder from PBS, PEG, sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O (15 minutes at RT).
Purity
Purified by thiophilic adsorption and size exclusion chromatography.
Specificity
Recognizes the free N-terminus of human Amyloid beta A4 polypeptide. Does not crossreact with Amyloid Precursor Protein (APP).
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