Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Highly purified recombinant human SAA.
Form
Supplied as a lyophilized powder in 0.01M PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes natural and recombinant human SAA at ~12kD. Does not show any crossreaction with other human cytokines or growth factors tested such as IL-1beta , IL-8, MCAF, TGF-beta and EGF.