Amyloid beta A4 Precursor Protein-Binding, Family B, Member 1

Clone Type

Polyclonal

Host

Rabbit

Source

Rat

Swiss Prot

P46933

Isotype

IgG

Grade

Affinity Purified

Applications

IP WB

Crossreactivity

Hu Mo Rt

Gene ID

322

Shipping Temp

Blue Ice

Storage Temp

-20°C

FE65 is a signaling adaptor of APP protein. The FE65/APP complex was shown to translocate into the cell nucleus and regulate gene expression. Genetic studies in mice validated FE65-APP interaction and molecular studies provided evidence on the role of the complex in abnormal signaling detected in brains of Alzheimer Disease patients.

Applications

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions

Western Blot: 1:1000, a band is seen at ~100kD versus the predicted molecular weight of 77kD, which corresponds to earlier findings in literature Immunoprecipitation: 2-10ug Optimal dilutions to be determined by the researcher.

Positive Control

IMR32 neuronal cell lysate, HEK 293 cell lysate, or brain lysate

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Recombinant protein corresponding to rat FE65, expressed in bacteria. Cellular Localization: Cytoplasmic

Form

Supplied as a liquid in PBS, 0.02% sodium azide, 15% glycerol.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes rat FE65. Species Crossreactivity: mouse and human

References

1. Zambrano, N., et al. Interaction of the Phosphotyrosine Interaction/Phosphotyrosine Binding-related Domains of FE65 with Wild-type and Mutant Alzheimer's beta-amyloid Precursor Proteins. JBC. 272:6399-405 (1997). [Western Blot, Immunoprecipitation] 2. Cao, X., and Sudhof, TC. A Transcriptionally active complex of APP with FE65 and Histone Acetylase TIP60. Science. 293:115-120 (2001). 3. Guenette, S., et al. Essential Roles for the FE65 Amyloid Precursor Protein-interaction Proteins in Brain Development. EMBO. 25:420-431 (2006). [Immunohisto/cytochemistry] 4. Muller, T., et al. Modulation of Gene Expression and Cytoskeletal Dynamics by the APP Intracellular Domain. MBC.

USBio References

No references available

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A2275-61P2 Rabbit Anti-Amyloid beta A4 Precursor Protein-Binding, Family B, Member 1 (APBB1, Fe65, MGC:9072, RIR, Adaptor Protein FE65a2, Stat-like Protein)

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