Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Western Blot: 1-4ug/ml. In IGF-1 stimulated LNCaP cells (passage number 38), a ~110kD band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 and Ser210. Variations in notation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen. Immunohistochemistry (paraffin): 1:10-1:500 Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Synthetic peptide corresponding to aa207-221 [GRAREApSGAPTSSKD] of human androgen receptor, containing the serine 213 phosphorylation site

Supplied as a liquid in PBS, 0.05% sodium azide.

Purified by Protein G affinity chromatography.

Recognizes human androgen receptor phosphorylated at Ser213/210