Angiopoietin-like 1 is a secreted glycoprotein structurally related to the angiopoietins. It contains an N-terminal coiled-coiled domain and a C-terminal fibrinogen-like domain. Unlike angiopoietins which are agonists and antagonists of the Tie-2 receptor tyrosine kinase, Angiopoietin-like 1 is an orphan ligand that does not bind Tie-2.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.2ug/mL Detection limit is ~2ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ala39-Asp491 from human Angiopoietin-like 1, expressed in NSO cells.
Form
Supplied as a lyophilized powder from PBS, 50ug of BSA. Label with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Angiopoietin-like 1. |Crossreactivity: rhAngiopoietin-4; <20%, rhAngiopoietin-1 and rhAngiopoietin-2; <1%
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