Applications
Suitable for use in Neutralization, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 50ug/ml ELISA: 0.5-1.0ug/ml Western Blot: 0.1-0.2ug/ml Immunohistochemistry: 2-15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived recombinant human Angiotensin I Converting Enzyme ectodomain aa30-1261.
Form
Supplied as a lyophilized powder in 0.2 um filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ACE ectodomain.