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A2295-07G Angiotensin II Type 1 Receptor, Rat, Control Peptide |(AT1) CAS:

Specifications
References
Grade
Highly Purified
Specificity
Synthetic peptide 10aa sequencemapping near the N-terminus of rat AT II (1). This peptide is predicted to be extracellular. Sequence is the same in all AT II receptor isoforms (Type 1a, 1b and 1c). No sequence homology with other G-protein coupled receptors.Species sequence homology: Mouse, rat, human, porcine and rabbit: 100%.
Applications
E
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

Control Peptide for A2295-07D (antiserum) and A2295-07E (affinity purified antibody).

Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2). Major cardiovascular effects appear to be mediated through AT1. AT1 has three isoforms (rat AT1A 359aa; AT1B/AT III, 359aa; and AT1C, 177aa). Rat AT1 receptors are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular. The C-terminus is inside the membrane (cytoplasmic). The binding of angiotensin II with AT1 receptors activate a phosphatidylinositol-calcium second messenger system. AT1 receptors are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle. AT2 gene ( chromosome x) encodes 363 aa protein. It is highly expressed in myometrium with lower levels in adrenal and fallopian tube. It is also expressed at high levels in fetal kidney and intestine.
Rat AT1 receptors are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular, while C-terminus inside the membrane (cytoplasmic). The binding of angiotensin II with AT1 receptors activate a phosphatidylinositol-calcium second messenger system. AT1 receptors are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle. AT2 gene (chromosome x) encodes 363aa protein. It is expressed at high levels in myometrium, fetal kidney and intestine. It is expressed at lower levels in adrenal and fallopian tube.
Applications
Suitable for use in ELISA, Antibody Blocking, Not suitable for use in Western Blot due to low moleculare weight. Other applications not tested.
Recommended Dilution
Antibody Blocking: 5-10ug per 1ul A2295-07D (antiserum) or 1ug A2295-07E (affinity purified antibody). ELISA: 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Rat synthetic peptide
Purity
Highly purified
Concentration
~1mg/ml
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Murphy, T.J., et al., Nature 351: 233 (1991). 2. Iwai, N., et al., BBRC 177: 299 (1991). 3. Langford, K., et al., BBRC 183: 1025 (1992). 4. Sasamura, H., et al., BBRC 185: 253 (1992). 5. Yoshida, H., et al., BBRC 186: 1042: (1992). 6. Mauzy, C.A., et al., BBRC 183: 8 (1992). 7. Bergsma, D.J., et al., BBRC 183: 989 (1992). 8. Konishi, H., et al., BBRC 199: 467 (1992). 9. Iwai, N., et al., FEBS Lett. 298: 257 (1992). 10. Sandberg, K., et al., BBRC 183: 1090 (1992). 11. Elton, T.S., et al., BBRC 184: 1067 (1992). 12. Hahn, A.W., et al., BBRC 192: 1260 (1993).
USBio References
No references available
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