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A2297-27 Antibiotic Medium #3 (Powder)

Specifications
References
Grade
Microbiological Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Penassay Broth

Dehydrated culture media suitable for the microbial assay and detection of antibiotics in milk and plate counts in pharmaceutical products. Formulated in acordance with the specifications and protocols as listed by the FDA, Grove and Randall and USP 23.

The activity of an antibiotic can be demonstrated under suitable conditions by its inhibitory effect on organisms. Antibiotic assays are performed by the cylinder plate method and the turbidimetric assay. These media has been formulated according to the specifications and procedures listed by the Food and Drug Administration, European Pharmacopeia, Code of Federal Regulations, and the USP.
Appearance
Light beige, homogeneous and free flowing powder
Solubility
Yellow to medium amber and turbid to clear
pH
As Reported
Directions per Liter
Dissolve 17.5 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown asg/liter
Beef Extract1.5
Dextrose1.0
Pancreatic Digest of Gelatin5.0
Potassium Phosphate, Dibasic3.68
Potassium Phosphate, Mono1.32
Sodium Chloride3.5
Yeast Extract1.5
Total per Liter:17.5g
References
1. CRF:21, 300.50 2. Bacteriological Analytical Manual, Sixth Ed. (1984) 3. Assay Methods of Antibiotics, Grove, D.C. and Randall, W.A., Medical Encyclopedia Inc., N.Y. (1955) 4. USP XXI, Rockville, MD (1985)
USBio References
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