Suitable for use in ELISA, Immunoelectrophoresis and Single and Double Radial Immunodiffusion. Other applications not tested.

Immunoelectrophoresis: 2ul plasma or equivalent against 120ul antiserum Double Radial Immunodiffusion: Use a rosette arrangement with 10ul antiserum in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells. Single Radial Immunodiffusion: 1% in the gel. Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Supplied as a lyophilized powder in PBS, pH 7.2. Preservative free. Reconstitute with 1ml sterile dH2O.

Serum

Recognizes human a1-Antichymotrypsin.