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A2298-11M Mouse Anti-Anti-Mullerian Hormone (AMH, Muellerian-inhibiting Factor, Muellerian-inhibiting Substance, MIS)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
10B151 (5/6)
Grade
Supernatant
Applications
IHC WB
Crossreactivity
Hu Mk Mo Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Anti-mullerian hormone (AMH) is originally classified as a fetal testicular hormone that inhibits Mullerian duct development. AMH is expressed post-natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth. AMH is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulfide linked precursor that is cleaved to release the mature 30kD homodimer.

Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Paraffin): 1:20-1:40, Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.
Positive Control Tissue
Ovary
Hybridoma
Sp2/0 myeloma cells with spleen cells from T/O outbred mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human Anti-Mullerian Hormone (VPTAYAGKLLISLSEERISAHHVPNMVATEC).
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Supernatant
Specificity
Recognizes human Anti-Mullerian Hormone. |Species Crossreactivity: mouse, sheep, Squirrel monkey and baboon.
References
1. Van Saen, D., et al., Hum Reprod. 26(2): 282-293 (2011). 2. Gruijters, M., et al., Mol. Cell. Endocrinol. 211: 85-90 (2003). 3. Weenen, C., et al., Mol. Hum. Reprod. 10(2): 77-83 (2004). 4. Papanastasopoulos, P., et al., Fertil. Steril. 92: 1169.e11-4 (2009). 5. Campbell, B.K., Anim. Reprod. 6: 159-71 (2009). 6. Walker, M.L., et al., Reproduction 138: 793-799 (2009). 7. Sobinoff, A.P., et al., Toxicol. Sci. 123(2):563-575 (2011). 8. Van Saen, D., et al., Hum. Reprod. 27(2): 323-330 (2012). 9. David, A., et al., Hum. Reprod. 27(4): 1088-1095 (2012). 10. Kevenaar, M.E., et al., Endocrinology 147: 3228-3234 (2006). 11. Campbell, B.K., et al., Endocrinology 153: 4533-4543 (2012). 12. Amorim, C.A., et al., Human Reprod. 28(8): 2146-2156 (2013).
USBio References
No references available
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