Serpin Peptidase Inhibitor, Clade F (alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 2
Apha-2 antiplasmin is a single-chain glycoprotein with a molecular weight of about 65000. The normal concentration in human plasma is 60mg/ml. Platelets contain significant amounts of the protein, which is released along with platelet factor 4, following thrombin stimulation. It can form 1:1 complexes with circulating plasmin. Alpha-2 antiplasmin is the most important inhibitor of plasmin-mediated fibrinolysis, but also capable of inactivating factor XIIa, kallikrein, factor XIa, thrombin and factor Xa. The importance of the alpha-2 antiplasmin as regulator of the fibrinolytic system was detected by the discovery of a congenital deficiency.
Applications
Suitable for use in use in ELISA, Bidimensional Electrophoresis, Immunoelectrophoresis and Double Radial Immunodiffusion (Ouchterlony). Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Alpha-2 antiplasmin isolated from pooled human plasma.
Form
Supplied as a lyophilized powder, 1mg/ml sodium azide. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity
Recognizes human alpha-2 antiplasmin. Does not crossreact with any other human plasma proteins. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen.
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