Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Highly purified porcine a1-antitrypsin isolated from pooled normal plasma

Supplied as a lyophilized powder. No preservatives added. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

Serum

Recognizes porcine a1-antitrypsin. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony) against fresh normal porcine plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified a1-antitrypsin. No reaction is obtained with any other plasma protein component or serum.