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A2298-74R Rabbit Anti-Atg7 (Autophagy Protein 7, Ubiquitin-activating enzyme E1-like protein, hAGP7, APG7-like, ATG7, APG7L) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
O95352
Isotype
IgG
Grade
Serum
Applications
IF IHC
Crossreactivity
Hu Rt
Accession #
XP_533740
Shipping Temp
Blue Ice
Storage Temp
-20°C

Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. Subunit: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. In yeast, ATG7 appears to be required for fusion of peroxisomal and vaculuolar membranes. Belongs to the ATG7 family.

Domain
The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
Tissue specificity
Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Applications
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:200-1:3000 Immunofluorescence: 1:200-1:3000 Optimal dilutions to be determined by the researcher. .
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Immunogen
Synthetic peptide corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein. CELLULAR LOCAlizaTION: Cytoplasm (Probable).
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Purity
Serum
Specificity
Recognizes human ATG7. Species crossreactivity: rat. Species sequence homology: mouse, rat, S. cerevisiae, Macaca mulatta, and Canis familiaris.
References
1. Yuan W, et al. Mol. Biol. Cell 10:1353-1366(1999). 2. Tanida I, et al. Biochem. Biophys. Res. Commun. 292:256-262(2002). 3. Tanida I, et al. J. Biol. Chem. 277:13739-13744(2002).4. Mizushima N, et al. Nature 395:395-398(1998). 5. Johnston M, et al. Science 265:2077-2082(1994). 6. Harding T.M, et al. J. Cell Biol. 131:591-602(1995). 7. Kim J, et al. Mol. Biol. Cell 10:1337-1351(1999). 8. Komatsu M, et al. J. Biol. Chem. 276:9846-9854(2001). 9. Klionsky D.J, et al. Dev. Cell 5:539-545(2003). 10. Chimura Y, et al. J. Biol. Chem. 279:40584-40592(2004).
USBio References
No references available
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