Cytoplasm.

2 named isoforms produced by alternative splicing.

Ubiquitous. Belongs to the ATG12 family.

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

A synthetic peptide corresponding to the N-terminal of human APG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.

Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.

Purified by affinity chromatography.

IHC and WB confirmed the specificity for APG12. Species Crossreactivity: Human, rat