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A2298-75D Rabbit Anti-Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
IF IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

APG10 plays a role in autophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule. SUBUNIT: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7.

Applications
Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immuhistochemistry (IF): 1:100-1:1000 Optimal dilutions to be determined by the researcher.
Cellular Localization
Cytoplasm (Probable).
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to the C-terminal of human APG10 protein
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Purity
Serum
Specificity
Recognizes human APG10. Species Crossreactivity: human
References
1. Wan D. et al. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729 (2004) ; 2. Ota T. et al. Nat. Genet. 36:40-45(2004) ; 3. The MGC Project Team. Genome Res. 14:2121-2127(2004).
USBio References
No references available
Conjugates
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