Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Immunofluorescence: 1:100-1:3000 Immunohistochemistry: 1:100-1:3000 Western Blot: 1:100-1:3000 Optimal dilutions to be determined by the researcher.

Cytoplasm. Preautophagosomal structure; preautophagosomal structure membrane; peripheral membrane protein. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide corresponding to the N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein.

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.

Serum

Recognizes human ATG16 L1. Peptide Sequence Homology: mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).