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A2298-89G Goat Anti-APOBEC3A (Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide 3A, APOBEC 3A, APOBEC-3A, ARP3, BEDP, bK150C2.1, Catalytic Polypeptide-like 3A, Phorbolin 1, PHO1, PHRBN)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Accession #
NP_663745.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
APOBEC3A; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A; ARP3; PHRBN; bK150C2.1; phorbolin 1

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 28kD band in Human Spleen, Tonsil and Peripheral Blood Mononucleocyte lysates after 0.1ug/ml antibody staining.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking
Corresponding peptide is available for Peptide Blocking studies. See A2298-89G-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Immunogen
A synthetic peptide from the C-terminus of aa--TSNFNNGIGRHKTY
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human Phorbolin 1.
References
Targeting APOBEC3A to the viral nucleoprotein complex confers antiviral activity., Goila-Gaur R, Khan MA, Miyagi E, Kao S, Strebel K., Retrovirology. 2007 Aug 29;4:61.
USBio References
No references available
Conjugates
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