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A2299-36L Mouse Anti-APOA4 (Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P06727
Isotype
IgG2a
Clone Number
1D4C11
Grade
Ascites
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NM_000482, NP_000473.2
Shipping Temp
Blue Ice
Storage Temp
-20°C

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000 Western Blot: 1:100-1:500 Immunohistochemistry (FFPE): 1:200-1:400. Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein fragment corresponding to aa21-396 of human APOA4 expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Recognizes human APOA4.
Conjugates
Pricing
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