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A2299-42J Goat Anti-Apolipoprotein M (ApoM, G3a, HSPC336, MGC22400, NG20, NG20-like protein)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_061974.2
Shipping Temp
Blue Ice
Storage Temp
-20°C
NG20, G3a, NG20-like protein, NG20, MGC22400, HSPC336, G3a, apolipoprotein M, APOM

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized.

Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:8000 Western Blot: 0.2ug/ml; ~50kD band in Human Kidney and Liver lysates Immunohistochemistry: 3-6ug/ml; In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to PRNQEACELSNN, from the C Terminus of human Apolipoprotein M (NP_061974.2).
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human Apolipoprotein M. Species sequence homology: canine.
References
1. Xu N, Nilsson-Ehle P, Ahren B. Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects.J Nutr Biochem. 2004 Oct;15(10):579-82.
USBio References
No references available
Conjugates
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