Applications
Suitable for use in ELISA, Western Blot, RIA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Immunocytochemistry: 1:100 Immunohistochemistry: Paraffin and frozen sections Optimal dilutions to be determined by the researcher.
Using conjugated L-Arginine-KLH, antibody specificity was performed with an ELISA test by competition experiments with the following compounds
Compound : Cross-reactivity ratio(a) L-Arginine-BSA: 1 SDMA-BSA: 1: >100 NDMA-BSA: 1: >100 ADMA-BSA: 1: >100
(a) L-Arginine-BSA concentration/unconjugated or conjugated amino acids concentration at half displacement
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
L-Arginine, conjuagted to KLH.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 20ul ddH2O.
Specificity
Recognizes L-Arginine. See additional crossreactivity data.