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A3578-10A Rabbit Anti-ART3, NT (Ecto-ADP-ribosyltransferase 3, NAD(P)(+)-arginine ADP-ribosyltransferase 3, Mono(ADP-ribosyl) Transferase 3, TMART)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q13508
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_001170
Shipping Temp
Blue Ice
Storage Temp
-20°C

Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367aa human ART3 protein has an estimated molecular mass of 41.5kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the N-terminal region of human ART3 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human ART3. Species Crossreactivity: mouse.
References
1.Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2.Koch-Nolte, F., et al., Genomics 39(3):370-376 (1997). 3.Levy, I., et al., FEBS Lett. 382(3):276-280 (1996).
USBio References
No references available
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