Autophagy-related Protein 9A, APG9-like 1, Cytoplasm to Vacuole Targeting Protein 7, Autophagy-related Protein 9, APG9A
Survival of environmental stress conditions requires the maintenance of cellular homeostasis. To preserve this balance, cells utilize a degradative mechanism known as autophagy. During this process, in response to starvation or other stresses, bulk cytoplasm is non-specifically sequestered within double-membrane vesicles and delivered to the lysosome/vacuole for subsequent degradation and recycling. Atg9 is the only integral membrane protein required for autophagosome formation and considered a membrane carrier in autophagy-related pathways.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Cellular Localization: Membrane; multi-pass membrane protein.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide made to a region within the C-terminus of the human protein (between residues 750-839).
Form
Supplied as a liquid in PBS, 0.02% sodium azide
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ATG9A protein (Mr ~95kD). Species Sequence Homology: 100% (monkey, mouse, rat, and cow); 94% (chicken)