Suitable for use in ELISA, RIA. Other applications not tested.
Recommended Dilution
RIA: 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic human pro-ANP (aa 31-67) polylysine conjugated|(EVVPPQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQR).
Form
Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Purity
Serum
Specificity
Recognizes synthetic human pro-Atrial Natriuretic Peptide (aa 31-67). There were no cross reactivities obtained with human pro-ANP (aa 67-98) and human pro-ANP (aa 1-30)
References
Hartter E, Khalafpour S, Missbichler A, Hawa G, Woloszczuk W. Enzyme immunoassays for fragments (epitopes) of human proatrial natriuretic peptides. Clin Chem Lab Med 2000;38:27-32.