Avidin D is isolated from egg white by a specific affinity system which results in a homogeneous, ultrapure avidin with very low nonspecific binding characteristics. Avidin D is recommended for use in solid-phase assays and many histochemical applications when the technique of sequential addition of reagents (“sandwich” technique) is employed.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 5-50ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sodium bicarbonate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Isolated from egg white
Concentration
Not Determined
Form
Supplied as a powder, no preservatives added. Reconstitute with 0.1M sodium bicarbonate, 0.09% sodium azide.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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