Avidin D, Fluorescein labeled, having an excitation at 495nm and an emission at about 515nm, is made from highly purified Avidin D by a special labeling technique. High fluorochrome to protein ratio and is the reagent we recommend for routine immuno-fluorescence applications. Compared to conventional primary and secondary fluorescent labeling techniques, Fluorescein Avidin D can provide greater sensitivity and lower background staining.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 5-30ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 8.5, 0.08% sodium azide. Labeled with Fluorescein.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.