Anthrax toxin plays a major role in the pathogenesis of anthrax. Virulent strains of Bacillus anthracis produce a complex exotoxin with 3 distinct antigenic components: the edema factor (EF), the protective antigen or binding domain (PA), and the lethal factor (LF).
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
B. anthracis spore extract.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes Bacillus anthracis spore antigen (92-94kD protein bands). No crossreactivity with B. anthracis vegetative cells, B. globigii, B. subtilis or B. cereus spores.