Appearance: Light greenish-beige, homogenous, free-flowing powder Solubility: Yellow to yellow-green, opaque, complete pH (RT ): 7.2 ± 0.2

Medium was prepared according to label directions with 5% sheep blood and inoculated with the organisms listed. Approximate inoculum: 1000fcu. Cultures were incubated aerobically at 30 ± 2ºC and examined for growth at 18-48 hours. Growth: Fair to good Reaction: Blue colonies with lecithin precipitate (halo) Growth: Suppressed to inhibited Reaction: N/A

Sterile egg yolk suspension: 50ml Polymyxin B (100,000 units; aqueous; filter-sterilized): 2ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter Suspend 41g of the medium in 950ml of purified water. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Autoclave for 15 minutes at 121ºC (15psi). Cool to 45-50°C. Aseptically add 50ml of a sterile egg yolk suspension and 2ml of a filter sterilized aqueous solution of polymyxin B.

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.