Applications
Suitable for use in ELISA, Western Blot, RIA, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): 1ug/ml using kidney sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic human β-Defensin 4 (aa 3-39)|(ELDRICGYGTARCRKK(-)CRSQEYRIGRCPNTYACCLRK)
Form
Supplied as a lyophilized powder in 50mM Tris, pH 7.4.
Purity
Purified from cell culture supernatant by Protein G affinity chromatography
Specificity
Recognizes human beta-Defensin 4 (epitope: aa3-39)