Nickerson Medium; Bismuth Sulfite Glucose; Glycine Yeast Agar;
Isolation of Candida sp.
BiGGY Agar was formulated by Nickerson. Bismuth sulfite inhibits bacterial growth without affecting the growth of Candida. Candida sp. reduce the bismuth sulfite and produce pasty brown to black colonies. C. albicans produce characteristic colonial morphologies and can be differentiated from other Candida species.
Appearance
Light beige, homogeneous, free flowing powder
Solubility
Gray-white, clear, complete after autoclaving
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter; Dissolve 45 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as | g/liter |
| Agar | 16.0 |
| Bismuth Ammonium Citrate | 5.0 |
| Dextrose | 10.0 |
| Glycine | 10.0 |
| Sodium Sulfite | 3.0 |
| Yeast Extract | 1.0 |
TDS
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