Biotin is used as a growth factor in mammalian cell culture as well as having numerous immunological purification roles in avidin/streptavidin-biotin binding mechanisms.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
BSA-Biotin, KLH-Biotin
Form
Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes biotin. Does not react with free BSA or free KLH