Biotin is used for mmunological purification in avidin/streptavidin-biotin binding mechanisms. Biotin, also known as vitamin H or B7, is a water-soluble B-complex vitamin which is composed of an ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring.
Applications
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:20,000- 1:200,000 Western Blot: 1:10,000- 1:40,000 Immunohistochemistry: 1:1000- 1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against anti-Peroxidase and anti-Biotin.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
TDS
NCBI
UniProt
Tech Support
Customer Service
