Cultivate a wide variety of bacteria, usually prepared with blood to observe hemolytic reactions.
Appearance
Light beige, homogeneous and free flowing powder
Solubility (plain)
Yellow-beige, trace to slightly hazy
Solubility (wIth blood)
Red, opaque
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 37 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Cool to 45-50°C and add 5-7% sterile defibrinated equine or sheep blood.
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.