These Blood Base media contain highly nutritious extracts or digests of beef. The beef products supply vitamins, carbohydrates, salts and other organic nitrogen compounds. Other peptones provide additional growth factors. Sodium chloride provides essential ions and electrolyte balance. Lowering pH to 6.8 enhances hemolytic reactions of streptococci.

Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.

Escherichia coli (ATCC 25922): Growth Staphylococcus aureus (ATCC 25923): Growth Beta Streptococcus pneumonia (ATCC 6305): Growth Alpha Streptococcus pyogenes (ATCC 19615): Growth Beta

Appearance: Light beige, homogeneous and free flowing

Solubility: Cherry-red and opaque..

pH: 6.8 ± 0.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dissolve 40grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).

3. Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium.

4. Cool to 45-50°C. Dispense into Petri dishes or tubes

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.